Abstract: The purpose of this experimental study was to determine Aflatoxins content of Iranian wheat and variations of them after storage. The store condition was canvas covered. In our study we selected Shahid Elmi Silo in Karaj, Iran. After evaluation silo, sampling plan was specified. Sampling was done in two steps from one of canvas covered stores with 25000 ton capacity, during one week on August and after 4 months storage, on December 2005. At the first, 50 samples were analyzed by HPLC to determine Aflatoxins content and then 50 samples were analyzed, by same method. The results showed that: at the first step of sampling, total Aflatoxin was detected just in three samples at amounts 0.23, 0.22, 0.53 ppb. At the second step total Aflatoxin was detected just in two samples at amounts 0.15 and 0.10 Ppb. According to the results of this study, contaminated wheat with aflatoxins was very low and the amount of contamination wasn�t increased during storage.