Authors : A.V. Charles-Rodriguez , J.E. Mauricio-Benavides , Y. Garza-Garcia , C.N. Aguilar , R. Rodriguez-Herrera , J. Rodriguez and J.C. Contreras-Esquivel
Abstract: Microbial marine enzymes can offer novel biocatalysts with properties such as high salt tolerance, hypoerthermostability, barophilicity and a more simple approach in large scale cultivation. Chitosanases are enzymes with importance as low molecular weight chitosans and chitosan-oligomers producers, which show innumerable applications in various fields, such as the biomedical, pharmaceutical, agricultural, biotechnological and food industry fields. However, the use of chitosanase is limited due to of its high-cost implications and potential unavailability in bulk quantities. In this study the production of bacterial chitosanases was evaluated in new isolated sources from Valley of Cuatrocienegas, Coahuila, Mexico. Specific culture media using chitosan-oligomers was used as a single source of carbon. Under these conditions, Aeromonas sp. produced 501 U L -1 of chitosanase activity and 220 U L -1 for Bacillus cereus, respectively. The crude chitosanase activity of culture supernatant was maximal on the 150 h of culture. Below 40�C, viscosity kinetics have indicated that chitosan is efficiently hydrolyzed into chitosan-oligosaccharides by Aeromonas sp and Bacillus cereus in a more rapid manner. SDS-PAGE of enzymatic extract of Aeromonas sp. has indicated a single band of ~ 20 kDa.
A.V. Charles-Rodriguez , J.E. Mauricio-Benavides , Y. Garza-Garcia , C.N. Aguilar , R. Rodriguez-Herrera , J. Rodriguez and J.C. Contreras-Esquivel , 2008. Chitosanase Production by a New Bacterial Sources. Research Journal of Biological Sciences, 3: 957-963.