Authors : M. Heidarpour , F. Mokhtari , S. Mirdamadi and A. Gharashi
Abstract: The probiotic effect of Bifidobacterium explains the popularity of these bacteria in different commercial products. Fermented dairy products are generally considered to be one of the most suitable vehicles for the administration of an adequate number of probiotic bacteria to the consumer. In this study the aim was focused to isolate the strains which may exist in traditional dairies for further use as probiotic strains. In order to achieve our goal and find local strains 100 samples were taken from a wide area of Iran. Total 43 isolated colonies which have been identified by selective culture media, biochemical tests and antimicrobial resistance to mupirocin and confirmed by enzymatic assay with F6PPK, were exposed to PCR assay using Bif164/Bif662 primers which amplified a 523 bp region of the 16SrRNA gene. 30 isolates out of 43 primarily isolated colonies, which match all of microbiological and molecular assays reported as species belonging to Bifidobacterium genus.
M. Heidarpour , F. Mokhtari , S. Mirdamadi and A. Gharashi , 2008. Isolation and Identification of Bifidobacterium sp. in Iranian Traditional Dairy Products. Research Journal of Biological Sciences, 3: 979-983.