Authors : Hossein Shahoon, Zahra Yadegari, Naser Valaie, Sareh Farhadi and Roya Hamedi
Abstract: Hydroxyapatite (Ca10 (PO4)6 (OH)2) is the major inorganic component of hard tissues, the best bio-active materials which is compatible with the bone tissue. In addition, Hydroxyapatite nanoparticles (nHA) have received enormous national attention in medical and dental applications recently, the ultimate fate of the nHA within the body is still unknown. Degradation products of nanomaterials are potentially cytotoxic. Thus, it is essential to assess biocompatibility before their usage in clinical applications. The purpose of this research was to evaluate the biocompatibility of nHA on Human Peripheral Blood Mononuclear Cells (HPBMCs). To evaluation of the biocompatibility of nano-sized, rod-like hydroxyapatite particles, HPBMCs were isolated and cultured on a 96 well plate. Cells were exposed to nHA at the following: 15.5, 31.25, 62.5, 125, 250, 500, 1000, 2000, 4000 and 8000 ppm after 2, 24, 48 and 72 h later for measuring the biocompatibility of material, MTT method was utilized. Measuring the photo, absorption was done by ELISA reader system at 570 nm which assigne the vitality of cell by the value of MTT absorption cells. None of the nHA experimented concentrations were toxic so, it seems that nHA biomaterial has acceptable compatiblity with HPBMCs.
Hossein Shahoon, Zahra Yadegari, Naser Valaie, Sareh Farhadi and Roya Hamedi, 2010. Evaluation of Hydroxyapatite Nanoparticles’ Biocompatibility at Different Concentrations on the Human Peripheral Blood Mononuclear Cells: An in vitro Study. Research Journal of Biological Sciences, 5: 764-768.