Authors : Xun Wang, Haifeng Liu, Ling Zhao, Han Dong, Chunyou Ning, Xuewei Li and Hengmin Cui
Abstract: To study the effects of crude polysaccharides from Poria cocos (PCCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture medium contained different concentrations of PCCP (0, 50, 100 and 200 μg mL-1) for 48 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Oil Red O staining on days 8 after treatment with culture medium supplemented with different concentration PCCP (0, 50, 100 and 200 μg mL-1). As the results, the different concentration PCCP (50, 100 and 200 μg mL-1) inhibited to some extent proliferation of 3T3-L1 preadipocytes when compared with the control. But the difference is not significant (p>0.05). And PCCP stimulated cell differentiation into adipocyte at lower concentration (50 and 100 μg mL-1) (p<0.05) while it inhibited cell differentiation into adipocyte at high concentration (200 μg mL-1) (p>0.05). It can be concluded from the results that the PCCP was involved in regulation of adipogenesis and lipogenesis.
Xun Wang, Haifeng Liu, Ling Zhao, Han Dong, Chunyou Ning, Xuewei Li and Hengmin Cui, 2011. Effects of the Crude Polysaccharides from Poria cocos on the Proliferation and Differentiation of 3T3-L1 Cells. Research Journal of Biological Sciences, 6: 597-601.