Authors : Mohammad-Ghasem Aminozarbian, Batool Hashemibeni, Ali Ghazalgoo and Fariba Heidari
Abstract: New endodontic cements have been formulated for a possible substitution of Mineral Trioxide Aggregate (MTA), although their properties such as genotoxicity must be evaluated in vitro before applying in human beings in detailed. The aim of this study was to compare the genotoxicity of Calcium Aluminate α-Aluminate Cement (CAAC), Calcium Aluminate α-Aluminate plus Cement (CAAC plus) and a mixture of wollastonite and CAAC cement (WOLCA) and Mineral Trioxide Aggregate (MTA) using human gingival fibroblast cells and single cell gel (COMET) assay. In this experimental in vitro study, MTA, CAAC, CAAC plus and WOLCA cement prepared in final concentrations of 1, 10, 100 and 1000 μg mL-1 then these materials were added to the human gingival fibroblast cells for studying their effect on DNA. Then, COMET assay was done for examining the cement genotoxicity. An automatic analysis system (COMET Assay) was used in order to calculate DNA damage. Tail moment (result of tail DNA/total DNA by the center of gravity) was considered to determine DNA damage. The potential DNA damage associated with exposure to different materials were calculated and statistically analyzed by Kruskal-Wallis and Mann-Whitney tests. DNA damage values for MTA were in 0.94-0.97 range; of CAAC in 0.96-1.02; of CAAC-Plus in 1.18-1.23 and of WOLCA was in 0.99-1.01 range. MTA had the least DNA damage values compared to others, although CAAC cement showed DNA damage similar to MTA. CAAC Plus and WOLCA had significantly higher genotoxicity than MTA, however the values obtained for CAAC cement was comparable to MTA suggesting its possible use in vivo.
Mohammad-Ghasem Aminozarbian, Batool Hashemibeni, Ali Ghazalgoo and Fariba Heidari, 2012. Genotoxicity of Mineral Trioxide Aggregate and Three Endodontic Cements on Human Gingival Fibroblast Cells. Research Journal of Biological Sciences, 7: 123-127.