Abstract: Several olive nurseries in Jordan were surveyed for basal and root rot fungi in olive cuttings during the rooting stage in Summer and Autumn 2011. Causal agents were isolated from symptomatic olive cuttings on PDA and identified according to specific keys. The most frequently isolated fungi were Pythium aphanidermatum followed by Fusarium solani, Rhizoctonia solani and Verticllium dahlia. Pathogenicity tests showed variation in the disease incidence caused by the isolated fungi on olive cuttings from different olive varieties. The highest disease incidence was caused by the fungus P. aphanidermatum on raseei olive variety (47%) were on other olive varieties, incidence ranged between 21 and 26%. The disease incidence caused by each of the other isolated fungi ranged from 11-15% on the different tested olive varieties. Var., raseei was the most affected by both P. aphanidermatum (47%) and composite sample (68%). Analysis of variance showed significant effects of nabali olive variety on disease incidence for the different fungal isolates.