Abstract: The aim of this study was to know the relation between HPV type 6 and 11 and the head and neck squamous cell carcinomas in Sudanese patients and to study the histopathology of those neoplasms. About 59 HNSCCs samples, including 28 previously HPV type 16 negative extracted DNA samples and 31 formalin fixed paraffin-embedded tissues were used for conventional polymerase chain reaction to detect HPV type 6 and 11. Statistics Package of Social Sciences (SPSS) was used to analyze the data. In those biopsies, there were 34 male squamous cell carcinomas (58%) and 25 female squamous cell carcinomas (42%). DNA of HPV type 6 and 11 was each detected in about 25% of the examined samples. The DNA samples that were HPV type 16 negative revealed 14 positive samples for HPV type 6 and total absence of HPV type 11. The age category 41-50 years was found to be the most affected age category with HNSCC in both genders. It constituted 27% of HPV type 6 positive samples and 13% of HPV type 11 positive samples. The most frequent site for HNSCC is the nasopharynx that constituted about 64% (38 sample) of the total examined samples, 53% of the HPV type 6 and 27% HPV type 11 positive samples were from that site. Most of positive samples for HPV type 6 were poorly differentiated. Most of positive samples for HPV type 11 were well differentiated. Most of positive samples for HPV type 6 were non-keratinized (60%) whereas 60% of HPV type 11 were keratinized. The relation between HPV type 6 and 11 and the gender age site of SCC, differentiation and keratinisation was found to be insignificant. Further studies are needed investigate the relation between HPV genotypes (HPV type 6, 11 and 16) to probably induce multiple infection required for the development of malignant lesions.
Hind El Rayah Osman, Ebtihal Hashim Bareer, Nazik Elmalaika and Obaid Seid Ahmed Husain, 2013. Association of Human Papillomavirus Type 6 and 11 with Head and Neck Squamous Cell Carcinomas in Sudanese Patients. Research Journal of Biological Sciences, 8: 174-178.