Authors : K.O. Oyedeji, A.F. Bolarinwa and O.A. Afolabi
Abstract: Tetracycline has been reported to be a broad-spectrum bacteriostatic autibiotics that inhibit protein synthesis but there is a dearth of information on its effect on blood chemistry and reproduction in female Albino rats. This study was designed to investigate the effect of this drug on haematological and reproductive parameters in female Albino rats. Tetracycline (5 mg kg-1 BW) was administered to the rats for 30 days for haematological and histopathological study and 21 days for estrous cycle study. Distilled water (0.5 mL) served as the control. Red Blood Cell (RBC) and Total White Blood Cell (TWBC) counts were determined using haemocytometer. Packed Cell Volume (PCV) was determine by micro-Haematocrit method. Differential leucocyte count was done using Schilling method. Vaginal smears were stained using the Papanicolaou’s staining technique. Routine histological technique was used in preparing the histological sections of the ovaries and uteri. Data were analysed using student’s t-test at p<0.05. Treatment of rats with tetracycline caused non-significant changes in RBC, TWBC, PCV and differential leucocyte values relative to their respective controls. Tetracycline caused significant decrease in the proestrous phase and a significant increase in the metestrous phase of the estrous cycle relative to their respective controls. However, it produced no pathological effect on the ovaries and uteri.
K.O. Oyedeji, A.F. Bolarinwa and O.A. Afolabi, 2013. Effect of Tetracycline on Haematological and Reproductive Parameters in Female Albino Rats. Research Journal of Pharmacology, 7: 12-15.