Abstract: This experiment was carried out to evaluation usage different levels of Canola Oil (CO) (0, 2 and 4%) and Choline Chloride Supplement (CCS) (0, 500 and 1000 mg kg ¹) in the basal diet (corn and soybean meal) and their effects on the broiler chick�s performance. This trial was conducted in 3 � 3 factorial experiment with Completely Randomized Design (CRD) with 270 male chicks (Ross 308). All diets were isoenergetic and isonitrogenous and balanced with NRC recommendation. Result shown that interaction effects of canola oil and choline chloride supplement could affected Live Body Weight (LBW), Feed Intake (FI) and Feed Conversion Ratio (FCR). In 22-42 day old canola oil and choline chloride supplement in 9 and 6 treatment (T9 = 4% canola oil + 1000 mg kg ¹ choline chloride supplement and T6 = 2% canola oil + 1000 mg kg ¹ choline chloride supplement) could significantly increase the live body weight, respectively (p<0.0001) and canola oil and choline chloride supplement in 8, 7 and 6 treatment (T8 = 4% canola oil + 500 mg kg ¹ choline chloride supplement, T7 = 4% canola oil + without choline chloride supplement and T6 = 2% canola oil + 1000 mg kg ¹ choline chloride supplement) could significantly increase the feed intake respectively (p<0.0001) and in 9 and 8 treatment (T9 = 4% canola oil + 1000 mg kg ¹ choline chloride supplement and T8 = 4% canola oil + 500 mg kg ¹ choline chloride supplement) could significantly decrease the feed conversion ratio respectively (p<0.0001). In 43-56 day old canola oil and choline chloride supplement in 6 and 4 treatment (T6 = 2% canola oil + 1000 mg kg ¹ choline chloride supplement and T4 = 2% canola oil + without choline chloride supplement) could significantly increase the live body weight (p<0.0001) and canola oil and choline chloride supplement in 1, 2, 4 and 7 treatment (T1 = basal diet, T2 = without canola oil + 500 mg kg ¹ choline chloride supplement, T4 = 2% canola oil + without choline chloride supplement and T7 = 4% canola oil + without choline chloride supplement) could significantly increase the feed intake respectively (p<0.0001) and in 9 and 8 treatment (T9 = 4% canola oil + 1000 mg kg ¹ choline chloride supplement and T8 = 4% canola oil + 500 mg kg ¹ choline chloride supplement) could significantly decrease the feed conversion ratio respectively (p<0.0001). No mortality was in all over period.