Abstract: Purpose of this study is to investigate the effect of information systemson supply chains with mediation of knowledge management in Iran Khodro Company in Isfahan. The said population for this study included all administrative staff in the dealerships of Iran Khodro in Isfahan who are serving during the year 2015. Totally, they are 460 persons. The sample size is 210 persons based on Morgan table. Questionnaires were distributed amongst administrative staff in the dealerships of Iran Khodro in Isfahan by random cluster sampling method, of which 210 questionnaires were analyzed. The research method was descriptive and correlational. In this study, three questionnaires were used including information systems in 19 items, supply chain in 18 items and knowledge management in 33 items based on the five-item Likert range. Validity of the questionnaire was confirmed by experts in management and its construct validity was confirmed by factor analysis and reliability was calculated using Cronbach’s alpha and for the supply chain is equal to 0.92, for the variable of information system is equal to 0.934 and for the variable of knowledge management is equal to 0.902.