Abstract: In the proposed context-based retrieval technique, a 2-DGE image is filtered out for noises and unobvious protein spots using a threshold. The significant protein spots on the filtered 2-DGE images will then be indexed and confined within their block properties which will all be stored in the 2DGE database for query purposes. Queries could be made by retrieving a 2-DGE image and marking one or multiple protein blocks where specific searches could be made to retrieve the required information. The process to create the 2-DGE database is simple and the search is quick. The proposed technique will be helpful to biologists with needs for unrestricted access to a 2-DGE image database.