Abstract: Injection is one of healthcare method. Injection can be used to curing and preventing but it has negative effect such as resulting in pain, overdose and spreading of infection. It can result in anxiety. This factorial design 2x2 study investigated the effect of thought stopping therapy in reducing anxiety of injection. Participants were 40 nursing student in Malang, Indonesia. They were 18-22 year old with 35-65 score of STAI-20 form Y2. Digital sphygmomanometer, digital thermometer and respiratory rate timer were used to measuring the change of physiological scored. Kruskal Wallis analysis showed that thought stopping therapy had significant effect in reducing anxiety of injection on blood pressure (p = 0.001) and pulse (p = 0.000) but this effects were not significant on temperature (p = 0.234) and respiratory rate (p = 0.55). This implies that stopping therapy can be used to reducing anxiety of injection.