Abstract: Swine fever is caused by the hog cholera virus (HCV), and is an important source of pathogen-related economic loss within the pig industry worldwide. Control of this disease is mainly by vaccination and commercial vaccines are not totally effective. DNA vaccine have many advantages over conventional vaccines, but handicap is its low efficacy .In this study, a new formulation of DNA vaccine was developed to improve its efficacy, results showed this new vaccine can enhance both humoral and cellular immune response compared with naked DNA vaccine.