Abstract: In this research, the effects of different breeding systems (Cage and Pond) on some important biochemical parameters (AkP Alkaline phosphates, TP Total Protein, GOT Glutamic oxaloacetic transaminase, GPT Aspartate aminotransferase and Cho Cholesterol) of rainbow trout were investigated. Comparing with the pond fish blood samples, the cage samples took higher AkP and GPT values but lower GOT, Cho and TP values.