Abstract: Aflatoxins are of serious concern for poultry industry and human beings as well. The deleterious effects of aflatoxins beyond the level of 20 ppb significantly inhibit the growth and productive performance of poultry. The present project was designed to study the effect of Aflatoxin B1 in various edible tissues of Gallus domesticus at the stage of their marketing. For this purpose liver, kidney, dressed meat and poultry feed of the representative flocks were collected, oven dried, their moisture contents and Aflatoxin B1 were determined through Thin Layer Chromatography. The data thus collected were statistically analyzed and the results showed that the Aflatoxin B1 level was significantly different in liver with respect to kidney and meat with minimum ratio of Aflatoxin B1.