Abstract: The objective of this study was to determine if the site of liver tissue collection would effect analysis of selected trace minerals in the liver of alpacas and llamas. Randomized post-mortem study. Livers were collected during post-mortem examination of alpacas and llamas that died of various diseases. Liver tissue was collected from the left, right, and caudate lobes of the liver for analysis. Trace mineral analysis was performed at a commercial laboratory using inductively coupled argon plasma emission spectroscopy. Significant differences were not found among sites of tissue collection except for potassium. Coefficients of variation were > 50% for calcium, copper, iron, molybdenum, selenium, and zinc. Site of liver tissue collection does not significantly effect trace mineral analyses in alpacas and llamas.