Abstract: In this study, it was aimed to determine the effect of adrenalectomy on the pregnancy rate and offspring numbers in rats. Three- month old, a total 90 virgin female albino rats (weighing 200-250 g) were caged. The animals were randomly divided into 5 groups. Unilateral adrenalectomy was applied to group1 (n=20) and group 2 (n=20) rats and bilateral adrenalectomy to group 3 (n=20) and group 4 (n=20) rats. Group 5 (n=10) was left without any application as a control group. One fertility proven male rat was placed into all cages of animals after 10 days of surgery. 2 g estradiol (SIGMA) and 5 mg progesterone (SIGMA) dissolved in 0.2 ml absolute alcohol was injected intramuscularly on the day when a male rat was put into cages of group 1 and 3 animals. Pregnancy of the animals was diagnosed and their delivery was followed up. Their offspring numbers were recorded on the day of delivery. In conclusion, it was determined that pregnancy rates were lower in group 3 and 4 rats that were undergone bilateral adrenalectomy than the other groups. However, no significant difference was observed with respect to offspring numbers among all groups.