Abstract: Cells of Pseudomonas aeruginosa when frozen at –20oC became injured. The study of the effect of high temperature on these freeze injured cells revealed that injured cells were sensitive to high temperature and they needed shorter time to lose their viability than non-injured cells. Also it was found that storage at -20oC of P. aeruginosa increased the lag phase time of the growth of this bacterium, and the injured cells became sensitive to sodium chloride and sodium nitrate. These two salts retarded the growth of injured cells. On the other hand sodium nitrite up to 800 ppm had no effect on injured cells.