Abstract: The fimbrial gene of Pasteurella multocida B:2, isolated from cattle with haemorrhagic septicaemia was amplified, cloned, sequenced and expressed in a vector. Cloning of the fimbrial gene revealed a 435 bp band while sequencing revealed similar nucleotide sequencing to the PtfA gene of P. multocida A:3 isolated from chicken, except the five nucleotide changes at residues 171, 282, 363, 387 and 414. The fimbrial gene was found to encode a deduced protein of 144 amino acids. The expressed fimbrial protein was detected from 4 h post-induction with a molecular weight of 18-kDa.