Abstract: The influence of two types of soluble non-starch polysaccharides (barley ?-glucan and maize arabinoxylan) at two inclusion levels (30 and 60 g/kg) on endogenous ileal nitrogen flow in broiler chickens was investigated, using the peptide alimentation method. Ileal nitrogen contents (as g/kg titanium marker) and endogenous nitrogen flow (as ?g/g dry matter intake) were numerically increased with the addition of non-starch polysaccharides, but the differences were not statistically significant (P>0.05). The results indicate that the type and level of soluble non-starch polysaccharides may influence the extent of ileal flow of nitrogen in chickens.