Abstract: Plasma concentrations of prolactin and cortisol were compared in twelve Native Karayaka sheep milked by machine versus nursed in early lactation. Sheep were divided into two groups. One group was machine milked; the other group was nursed without milked. The experiment was carried out first 30 day of lactation. Blood samples were taken at the end of the study and were assayed for hormones. Similar prolactin and cortisol were released in groups. There were no significant differences the plasma prolactin, cortisol and milk yields between groups. In early lactation period of Karayaka sheep there were no significant correlations among body weight, milk yield plasma prolactin and cortisol. Present data show that machine milking and nursing in sheep results stabled release of prolactin and cortisol in early lactation.