Abstract: During this study swab samples were tested for demonstration of Infectious Bovine Rhinotracheitis/ Infectious Pustular Vulvovaginitis (IBR/IPV) virus (BHV-1) from different regions of the Sudan. Samples were collected from cases suffering from infertility, nasal discharges, vaginal discharges, lacrimation and abortion. The virus was isolated from five cases with typical signs of the disease including two from Niyala, two from Atbara and one from West Kordofan. Also using SNT, ELISA and PHA testes neutralizing antibodies were found, which confirmed the presence of the disease.