Abstract: During serosurveillance to detect infectious bovine rhinotraceitis antibodies, 856 serum samples were collected from different states of the Sudan. The overall percentage of positive samples was 47.43% (406 samples) tested by PHA. Of 273 samples tested by ELISA 141 (51.65%) were positive, whereas 69 out of 94 samples tested by SNT 73% were positive. The prevalence was found high in states including Jongeli, West and North Kordofan. In comparison between the three serological tests, SNT is recommended to be the technique of choice followed by ELISA, while PHA is a good technique to define the disease.