Abstract: Intramuscular administration of vandyl sulphate at a dose of 10 and 20 mg kg 1 body weight for 6 days in sheep serum glucose levels at 50-53 mg dL 1, compared to 60-71 mg dL 1in their respective controls. Performance of glucose tolerance test indicated that total amount of glucose administered got utilized in 3 h in vandyl treated animals compared to 6 h in control. Vandyl sulphate used in the above specified doses was well tolerated in sheep.