Abstract: The present seroprevalence of EDS-76 by HI test revealed widespread distribution of EDS-76 antibodies in layer chicken in Kerala, India. Out of 615 sera samples tested by HI test, 58 samples found to be positive for EDS-76 virus antibodies. The overall prevalence of EDS-76 antibodies in Kerala was 9.43 % The attempts for virus isolation from clinical materials were failed.