Abstract: Macrolide Endectocides (ME) are antiparasitic agents that are daily applied in pharmacotherapy of food-producing animals. Worldwide numbers of pharmacokinetics studies performed on various matrixes of domestic animals with diverse detection methods for ME determination were developed. However only few studies on deer matrixes were published although ME are licensed and in use for treatment of red deer, fallow deer and reindeer in some countries of European Union, America and Australia. The purpose of this study was to follow some pharmacokinetic parameters of abamectin in fallow deer plasma 48 h after subcutaneous application of therapeutic dose. Biochemical and haematological parameters were also followed for 120 h after application of antiparasitic agent.