Abstract: The purpose of this study was to develop a technique of paralumbar fossa laparoscopic ovariectomy in 6 standing female donkeys using titanium clips and monopolar electrocautery and to avoid a laparotomy incision for ovary removal. Bilateral laparoscopic ovariectomy was successfully done in the standing donkeys using monopolar electrosurgical unit. This technique was found to be minimally invasive, can be performed efficiently and provided excellent haemostasis of the mesovarium.