Abstract: In the present study, normal values of serum non-electrolytes were studied in Makuei sheep breed. A total of 248 samples from 5 age groups and each sex were selected and measured of serum non-electrolytes including (glucose, cholesterol, triglyceride, BUN, uric acid, total protein, albumin and creatinine) carried out with spectrophotometric method. According to the results of present research, there was only a significant difference between two sex concerning BUN and triglyceride values and between age groups concerning cholesterol, uric acid, BUN and total protein values (p<0.05), also there was a significant correlation between age groups concerning cholesterol, total protein and BUN levels (p<0.05).