Abstract: To investigative the effect of dietary L-carnitine on ostrich sperm quality, a research was conducted in a completely randomized design consisting of 3 treatments and 4 replicates. The total numbers of birds used in this trail was 12 African black neck male ostrich. The experimental rations were prepared using the different levels (0, 250 and 500 ppm). The metabolizable energy, protein and the nutrient of these rations were similar. In order to study semen volume, sperm motility, live sperm percent, sperm count and abnormal sperm percent, after one month adaptation, semen collected at the beginning of every three month. The results of statistical analysis of data obtained indicated that there were significant effect of L-carnitine on semen volume (p<0.01), sperm motility, live sperm percent, sperm count (p<0.05) but L-carnitine had no significant effect on abnormal sperm percent.