Abstract: The use of sophisticated techniques for the analysis and identification of microorganisms is more and more common; such is the case of capillary zone electrophoresis technique, which allows a quick separation and identification of microorganisms. Actinobacillus seminis is a bacteria involved in ovine epididymitis, a disease common to this species and currently, its identification is solely bacteriological and is conducted through a microbiological analysis, which is confusing and slow, since there is not specific biochemical test that distinguishes this bacterium from other bacteria that are also involved in ovine epididymitis, such as Brucella ovis, mainly. This work presents pre-treatment results and run conditions of Actinobacillus seminis for its identification through capillary zone electrophoresis.