Abstract: Urine samples collected aseptically from the urinary bladders of 36 slaughtered indigenous camels were examined for leptospirosis by dark field microscopy and culture; all samples were negative. On the other hand, out of 90 Saudi camels' tested serologically for leptospirosis using the plate agglutination test, 6 (6.7%) were positive for leptospiral antibodies (L. autumnalis). This is the first serological record of camel leptospirosis in Saudi Arabia.