Abstract: Studies on functions of ovine granulocytes require pure and functionally active population of neutrophils. Because of lymphocyte contamination, common methods of sheep neutrophil isolation from peripheral blood sample do not result in an acceptable degree of purified live cells. So, based on neutrophil characteristics of sheep this study proposed a new and easy method of neutrophil isolation using Meglumine compound with the least deformity, high purity (79.5%) and high viability (>97.5%) of isolated cells.