Abstract: Canine Herpesvirus-1 (CHV-1) is an Alphaherpesvirus, the infection was detected throughout the world in dog population. In this study, 110 blood serum samples obtained from clinically healthy adult Kangal dogs, which are unvaccinated for CHV-1 in 4 private Kangal breeding kennels at Ankara province. The samples was controlled using indirect ELISA, as result of the test, out of 110 dog sera, 79 (71.8%) was found to be seropositive for CHV1. Positivity was found to be in all of the studied farms among 42.8 and 86.6% proportions. The important differences were not detected between male (21/32, 65.6%) and female (58/78, 74.3%) dogs as statistically. According to kennel records, no clinical findings related to the CHV1 infection were reported in any of the animals before, despite very high infection rates.