Abstract: This study was conducted to investigate the effects of heating and ventilation devices mounted to insulated and wooden bee hives on some physiological characteristics of honeybees. The average number of frames in groups Control (C), Heated (H) and Heated-Fan (HF) were 15.14±4.73, 16.98±6.05 and 18.74±7.34 colony-1, respectively. The average brood area for the colonies in groups C, H and HF were 4270.64±302.12, 4819.00±348.50, 5270.00±344.33 cm2, respectively. The values of flying activity in groups were 74.79±19.80 in C, 85.36±27.34 in H and 101.43±16.78 in HF. Honey yields in groups C, H and HF were 14.61±1.71, 20.63±1.99 and 28.14±2.97 kg colony-1, respectively.