Abstract: Two different vaccination programs against Newcastle Disease (ND) in Nineveh province was studied. Results showed that the first group, which vaccinated (live and inactivated) NDV with interval days was give high antibody titres at 18th days post vaccination with significantly difference (p<0.05), in comparison with the second vaccinated group that has NDV (live and inactivated) as well as live IBDV the antibody titres was significantly difference (p<0.05) at 28th days post vaccination. Serological antibody titres were determined to study the correlation between the 2 different vaccinated groups using ELISA test. It was concluded that the 2 different vaccinated programs show different immune response to ND.