Abstract: Twenty one clinical and sub-clinical sheep abscess disease isolates of Staphylococcus aureus subsp. anaerobius in addition to two reference strains were analysed for polymorphism of X region of protein A (spa) and staphylocoagulase (coa) coding genes. Two genotypes were obtained for spa gene: all of the 21 Sudanese isolates in addition one reference strain being located in one genotype. With coa gene, three genotypes were obtained: one genotype contained all of the Sudanese isolates; each of the two reference strains stood alone in its own genotype.