Abstract: Adipose depotsite-specific variability in physiological characteristics of preadipocytes could be in part, intrinsic to the cells and become evident through in vitro proliferation and differentiation-dependent gene expression patterns in preadipocyte in the cell culture. This study was designed to characterize proliferation and differentiation-dependent gene expression patterns intrinsic to intramuscular, perirenal and subcutaneous preadipocytes. About 3 type preadipocytes are prepared using the ceiling culture method from bovine intramuscular, perirenal and are demonstrated subcutaneous adipose depots of a single Japanese Black steer. Adipose depot site-specific proliferation and differentiation-dependent gene expression patterns intrinsic to preadipocyte by comparing the mRNA levels of CCAAT enhancer binding protein C/EBPα, C/EBPβ, C/EBPδ, Peroxisome Proliferator-Activated Receptor γ (PPARγ) and leptin before and after the induction of differentiation among culture of the 3 type preadipocytes. These results may suggest the existence of the site-specific preadipocyte-intrinsic proliferation and differentiation program.