Abstract: Twenty four Holstein cows in early lactation (days in milk: 24±4) were allocated equally to one of four treatments. Treatments were as follows: control basal diet without supplement; LY basal diet supplemented with 10 g of live yeast; ANX basal diet supplemented with 30 g of Aspergillus niger extracted meal and LY+ANX basal diet supplemented with 10 g LY and 30 g ANX. Daily individual milk production and feed intake recorded and weekly milk samples were taken for milk composition analyse. Fecal grab samples were collected during 3 consecutive days at the end of experiment period from each cow. The experimental period lasted 75 days. Milk production was improved by 6.8% for group fed the LY compared control diet (p<0.05). Fat-corrected milk, feed efficiency, body weight and body condition score changes and DM intake were similar between groups. Greater protein yield and percentage were observed in the LY and LY+ANX groups compared to the control group (p<0.05). No differences were observed in other milk composition between groups. The apparent digestibility of DM and OM were higher (p<0.05) in the LY+ANX supplemented diet compared to the control. Similar to DM and organic matter digestibility, greater NDF apparent digestibility was observed in the LY+ANX supplemented diet versus the control that might be due to synergistic effects of feeding LY and ANX together. We concluded that LY might have potential as an effective direct-fed microbial to increase milk production in early lactating cows.