Abstract: This study was conducted to evaluate the effects of adding levels of dried whey powder and probiotic to practical-type diets on the performance and Intestinal micro organism of laying hen’s. Dried whey was used in isocaloric and isonitrogenous diets at levels of 0, 2.5, 5 and 7.5% with two Level of probiotic (0 and 1000 g-1 Bioplus 2B ton-1 feed providing 0 and 3.2x106 cfu g-1 Feed concentration). About 72 white leghorn Hy-Line, w-36 strain used in experiment. Adding dried whey powder and probiotic to the diets caused highly significant increase (p<0.05) in egg production and lactobacillus in ileum but significant decrease (p<0.05) coliform in ileum. Using dried whey and probiobic improve egg weight, egg mass, shell thickness, shell hardness and Haugh unit but not significant (p>0.05). Level of 5% dried whey had significant decrease feed conversion (p<0.05). Other traits did not show significant different by using dried whey and probiotic.