Abstract: A study was conducted to evaluate the effects of addition different levels of probiotic to practical-type diet on the performance, egg quality and blood parameters of laying hens. The levels of probiotc was (0, 1000 and 2000 g Bioplus 2B ton-1 DM which provide 0, 3.2x106 and 6.4x106 cfu g-1 bacteria in feed). Twenty-seven Hy-Line W-36 strains of white Leghorn laying hens were assigned by a completely randomized design to 3 treatments with 3 replicates for each. Addition probiotic to the diets caused to significant increase (p<0.05) of egg production and egg mass and significant decrease (p<0.05) of feed conversion ratio. Probiobic improved egg weight, shell hardness and Haugh unit but not significant (p>0.05). Probiotic supplementation significantly decrease (p<0.05) the cholesterol and triglyceride of plasma also yolk cholesterol. Generally, the results showed that using 1000 g ton-1 probiotic had the best results than level of 2000 g ton-1.