Abstract: The content of Intramuscular Fat (IMF) of Anka chicken, Wenchang chicken and Rugao chicken from 4-12 weeks old were tested in this study. The results showed that Intramuscular fat content in three chicken breeds increased with the age. Herein, the IMF of breast muscle in Anka chicken sharply increased after 8 weeks old and was higher than that of Wenchang and Rugao chicken. But, there was no significant difference between different breeds before 8 weeks old. The IMF of hen is higher than that of cock in all three breeds, but only in Wenchang chickens the significant difference were detected between different sex.