Abstract: The effect of the duration of simulated transportation on blood SOD (Superoxide Dismutase), GSH-Px (Glutathione Peroxidase), AST (Aspartate Transaminase), ALT (Alanine Transaminase), GGT (Gamma-Glutamyl Transpeptidase), LDH (Lactic Dehydrogenase) enzyme levels and NO (Nitric Oxide) and MDA (Malondialdehyde) product levels was investigated in Japanese quails. Simulated transportation was carried out with a road simulator which was calibrated for Turkish roads conditions. Animals were loaded into well ventilated carrier boxes and carried at 2 and 5 h on the simulator. Blood samples were collected from 10-weeks-old Japanese Quails just after transportation. A significant (p<0.05) increase in all measured plasma parameters (except GGT) at 5 h transportation was observed. However, all of the plasma parameters have not shown a significant increase at 2 h transportation. This transportation duration might be the evidence of the time duration in still safe zone for Japanese Quails for that condition.