Abstract: The current study aiming at Raeini goat population in Iran based on microsatellite markers was undertaken. Genetic variation at 13 microsatellite loci was examined in this goat. All of 13 loci (LSCV36, TGLA122, MAF64, oarFCB304, oarJMP23, oarAE133, BM121, BM4621, ILSTS005, ILSTS022, ILSTS029, ILSTS033 and ILSTS34) were amplified successfully. The objectives of this study were to assess the genetic variability among Raeini goat populations. The genetic characterizations of this genetic resource are essential to conservation and breeding programs. The average number of alleles observed across the studied microsatellite loci was 7.84 and that of effective alleles was 5.38. The average expected heterozygosity values were 0.805. The mean polymorphic information content value (0.778) further reflected high level of polymorphism across the loci. Hardy-Weinberg Equilibrium (HWE) had been tested based on likelihood ratio for different locus by POPGENE software.