Abstract: This study aimed to determine the seroprevalence of Toxoplasma gondii infection in cattle from five localities in the province of Kars, Turkey. A total of 216 cattle serum samples were tested using an in-house ELISA, which was developed and optimised using sonicated tachyzoite antigens of RH strain T. gondii: 202 of the samples were found to be seropositive (93.5%). This result, which is consistent with findings from horses and sheep in previous studies undertaken in the area that T. gondii is highly prevalent in cattle in the region.