Abstract: An apparatus and method that can allow continuous and precise total collection of urine under normal conditions was developed. This apparatus and method satisfies the urine sampling requirement of balance studies and metabolic experiments of steers. The apparatus is comprised of a urine cup and collection barrel. The total and precise collection of urine can be achieved without leakage, fecal contamination and urethra infection. The apparatus has minimal negative effects on the steers and does not influence the steers normal physiological activities.