Abstract: The goal of the present epidemiological research was the detection of anti-Neospora caninum antibodies in order to determine the presence and prevalence of neosporosis in dairy cattle herds at 8 counties from the state of Nuevo Leon, Mexico. A total of 33 herds and 371 animals were included. Detection of Neospora caninum was performed using a commercial kit observed prevalence was 45%. This result allowed the conclusion that animals which are seropositive for the protozoan Neospora caninum do exist in Nuevo Leon. Serological evidence was found in animals at milk-production stage indicating that the presence of emerging diseases in the region is a fact therefore, sanitary measurements should be re-evaluated or reinforced in order to keep the cattle free from pathogens that have a negative impact in the production of food from animal origin used for the human consumption.