Abstract: The aims of this study was to evaluate efficacy of detecting male infertility in dogs using conventional Computer Assisted Sperm Analysis (CASA) for morphological analysis of spermatozoa and the Sperm Chromatin Structure Assay (SCSA) for level of DNA fragmentation in the spermatozoa. This study found percentage of morphological abnormalities and motility in infertile dogs were statistically significantly poorer than those in healthy control (p<0.05). Also percent DNA Fragmentation Index (DFI%) were significantly higher in infertile dogs. There is correlation between morphological mortality indices and DFI%. This result strongly suggested that assays such as SCSA could be a good alternative test for canine male infertility and for evaluating quality of sperm before artificial insemination as reported previously.