Abstract: The aim of this study is to provide insight into boron metabolism and to identify metabolic pathways which may explain the presumed increased susceptibility of livers. Boron was administrated in rabbits at three different doses and 96 h intervals for 7 months. Metabolomic profile based on NMR analysis was performed. The most pronounced findings were significant changes in alanine, methionine, pyruvate and creatine. Boron seems to be effective in the prevention of obesity and fatty liver. Metabolic end-points obtained by NMR can be easily assessed and interpreted alone or in combination each other and with classical biochemical parameters for better understanding obesity and boron and liver metabolism.