Abstract: This study was conducted to evaluate the effect of dietary vitamin C (Ascorbic Acid, AA) on reproductive performance of a freshwater ornamental species, the platy (Xiphophorus maculatus). Vitamin C was incorporated in fish feed at 6 different concentrations of 0.0 (A), 0.1 (B), 0.2 (C), 0.4 (D), 0.8 (E) and 1.6 (F) g kg-1 of dry diet with 5% of their body weight daily in two split doses for a period of 20 weeks. Results indicated that Gonadosomatic Index (GSI), fry production and relative fecundity were significantly higher in group F as compared to the control group (A) and other experimental groups (p<0.05). There were no significant differences in the weight and length of fry among the dietary treatments (p>0.05). The results also showed that the percentage of deformed fry was significantly lower in experimental groups (except the group B) compared to the control group (p<0.05). Moreover, the highest and lowest fry survival (%) were observed in group F and A, respectively. Collectively, this study showed that female broodstocks benefit from inclusion of vitamin C in diet during their reproductive stages.